Difference between revisions of "Part:BBa K419003:Experience"
(→Applications of BBa_K419003) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K419003=== | ===Applications of BBa_K419003=== | ||
| + | '''Verification of the Part BBa_k419003''' | ||
| + | |||
| + | [[Image:photo7.jpg]] | ||
| + | |||
| + | The DNA fragment of LuxR responsive promoter, LuxPR, was amplified from the part BBa_R0062 by PCR method. The LuxPR PCR products were purified and digested with EcoRI and PstI, then ligated with EcoRI-PstI cut pSB1C3, the iGEM 2010 standard backbone. We got the pSB1C3-LuxPR plasmid successfully. In addition, the lysis device DNA which contained holing and lysozyme genes was amplified from the part BBa_K112021. The lysis device PCR products were digested with Xba I and Pst I and ligated with the Spe I-Pst I treated pSB1C3-LuxPR vector. After transformation and plasmid preparation, the part BBa_k419003 for releasing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the lysis device DNA fragment. The releasing system composite was transformed into DH5 E. coli for further experiment. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:52, 6 November 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K419003
Verification of the Part BBa_k419003
The DNA fragment of LuxR responsive promoter, LuxPR, was amplified from the part BBa_R0062 by PCR method. The LuxPR PCR products were purified and digested with EcoRI and PstI, then ligated with EcoRI-PstI cut pSB1C3, the iGEM 2010 standard backbone. We got the pSB1C3-LuxPR plasmid successfully. In addition, the lysis device DNA which contained holing and lysozyme genes was amplified from the part BBa_K112021. The lysis device PCR products were digested with Xba I and Pst I and ligated with the Spe I-Pst I treated pSB1C3-LuxPR vector. After transformation and plasmid preparation, the part BBa_k419003 for releasing system in our Neutrient Synthesizer was verified by EcoRI and PstI enzyme digestion in 1% agarose gel electrophoresis. The arrow (red) indicates the lysis device DNA fragment. The releasing system composite was transformed into DH5 E. coli for further experiment.
User Reviews
UNIQc96586ebff99dc52-partinfo-00000000-QINU UNIQc96586ebff99dc52-partinfo-00000001-QINU