Difference between revisions of "Part:BBa K389004"

Revision as of 14:46, 26 October 2010

Luciferase from pGL4.10[luc2]

Structure of Photinus pyralis firefly luciferase. Image taken from: http://en.wikipedia.org/wiki/Luciferase.

A firefly (Photinus pyralis) luciferase from Promega's pGL4.10[luc2] vector. Luciferase is a sensitive reporter gene.

Usage and Biology

The activity of a luciferase is measured in a luminometer - so only use this BioBrick if you have one in your lab.

Firefly luciferase oxidizes its substrate, firefly luciferin, in presence of ATP and oxygen, emitting light ([http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VSR-4CJB7RR-7&_user=2459438&_coverDate=03%2F31%2F1996&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000057302&_version=1&_urlVersion=0&_userid=2459438&md5=ac60aa60eaad17b2aa1c4b31f954d0a8&searchtype=a Conti et al., 1996]). Because the firefly luciferin does not pass the cell membrane of E. coli under "normal" cultivation conditions the cells have to be lysated before the luciferase activity measurement.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 64
Illegal NgoMIV site found at 1408
Illegal NgoMIV site found at 1429
Illegal AgeI site found at 1132
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1314

@@ Line 13: / Line 13: @@
 The activity of a luciferase is measured in a luminometer - so only use this BioBrick if you have one in your lab.
-Firefly luciferase oxidizes its substrate, firefly luciferin, in presence of ATP and oxygen, emitting light (Conti ''et al.'', 1996). Because the firefly luciferin does not pass the cell membrane of ''E. coli'' under "normal" cultivation conditions the cells have to be lysated before the luciferase activity measurement.
+Firefly luciferase oxidizes its substrate, firefly luciferin, in presence of ATP and oxygen, emitting light ([http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VSR-4CJB7RR-7&_user=2459438&_coverDate=03%2F31%2F1996&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000057302&_version=1&_urlVersion=0&_userid=2459438&md5=ac60aa60eaad17b2aa1c4b31f954d0a8&searchtype=a Conti ''et al.'', 1996]). Because the firefly luciferin does not pass the cell membrane of ''E. coli'' under "normal" cultivation conditions the cells have to be lysated before the luciferase activity measurement.
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