Difference between revisions of "Part:BBa K358021:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K358021=== | ===Applications of BBa_K358021=== | ||
| + | ====Characterization of lytic activity with time==== | ||
| + | To characterize the lytic activity of our lysisbox if it would work or not as we expected, we focused on when cell lysis occurs after induction by IPTG. We did the experiment as this protocol, and we got the following data. | ||
| + | Protocol | ||
| + | # Pour 3mL of supplemented M9 medium to a Falcon tube. | ||
| + | # Pick out a colony on the plate of E.coli and put into the Falcon tube. | ||
| + | # Grow it at 30 degreees for 16h. | ||
| + | # Dilute it 50 folds with the same media and incubate until OD550 is about 0.15. | ||
| + | # Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG. | ||
| + | # Incubate the cultures at 30 degrees and measure OD550 of them. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 10:15, 6 November 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K358021
Characterization of lytic activity with time
To characterize the lytic activity of our lysisbox if it would work or not as we expected, we focused on when cell lysis occurs after induction by IPTG. We did the experiment as this protocol, and we got the following data. Protocol
- Pour 3mL of supplemented M9 medium to a Falcon tube.
- Pick out a colony on the plate of E.coli and put into the Falcon tube.
- Grow it at 30 degreees for 16h.
- Dilute it 50 folds with the same media and incubate until OD550 is about 0.15.
- Ditribute 3ml of the culture to falcon tubes and add certain amount of IPTG.
- Incubate the cultures at 30 degrees and measure OD550 of them.
User Reviews
UNIQ0ed8e6b9dc8daad5-partinfo-00000000-QINU UNIQ0ed8e6b9dc8daad5-partinfo-00000001-QINU