Difference between revisions of "Part:BBa B0034"
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RBS based on Elowitz repressilator. | RBS based on Elowitz repressilator. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | == IIT Madras 2016's Characterization == | ||
| + | [[Image:Nonmodularity_of_B343230.png|400px|right|Characterization of popular BioBrick RBSs]] | ||
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| + | == Modelling == | ||
| + | <p>Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts modelling]</p> | ||
| + | == Experimentation == | ||
| + | <p>Biobrick RBSs [https://parts.igem.org/Part:BBa_K1956022 B0030], [https://parts.igem.org/Part:BBa_K1956023 B0031], [https://parts.igem.org/Part:BBa_K1956014 B0032], [https://parts.igem.org/Part:BBa_K1956015 B0034] were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.</p> | ||
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Revision as of 21:42, 16 October 2016
RBS (Elowitz 1999) -- defines RBS efficiency
RBS based on Elowitz repressilator.
Usage and Biology
IIT Madras 2016's Characterization
Modelling
Global non-modularity towards promoters & protein coding parts and relative strength was estimated for RBSs B0030, B0032, B0034 in our [http://2016.igem.org/Team:IIT-Madras/Model#Modularity_of_RBS_parts modelling]
Experimentation
Biobrick RBSs B0030, B0031, B0032, B0034 were used in our 'Noise in Device' experiment to understand the role of RBS parts in noise.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| biology | -NA- |
| efficiency | 1 |
Note: The Elowitz RBS is the definition of efficiency 1.0.