Difference between revisions of "Part:BBa K299027"

 
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<partinfo>BBa_K299027 short</partinfo>
 
<partinfo>BBa_K299027 short</partinfo>
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This part contains RBS J61107 from the Anderson's collection.
 
This part contains RBS J61107 from the Anderson's collection.
  
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In the physical DNA after J61107 in composite parts there is Cactag sequence instead of the biobrick scar Tactag. J61101 was originally designed in a way  that its last nucleotide "C" is also first nucleotide of the suffix (wchich should be T)- it modifies the suffix. The sequence of J61101 in the registry does not include its last nucleotide. If it were included the registry sequence would show the additional nucleotide, now it shows T instead of C.
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In the physical DNA after J61107 in composite parts there is Cactag sequence instead of the biobrick scar Tactag. J61101 was originally designed in a way  that its last nucleotide "C" is also first nucleotide of the suffix (wchich should be T)- it modifies the suffix. The sequence of J61107 in the registry does not include its last nucleotide. If it were included the registry sequence would show the additional nucleotide, now it shows T instead of C.
  
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The seguence of the J612101 does not match its physical DNA
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The seguence of the J612107 does not match its physical DNA
  
 
To go around this problem in out bricks we used scarless assembly option and manually included the scars:
 
To go around this problem in out bricks we used scarless assembly option and manually included the scars:
  
 
BBa_K299800 DNA standard biobrick scar (if next part starts with ATG)
 
BBa_K299800 DNA standard biobrick scar (if next part starts with ATG)
 +
 
BBa_K299801 DNA standard biobrick scar (if next sequence does NOT start with ATG)
 
BBa_K299801 DNA standard biobrick scar (if next sequence does NOT start with ATG)
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BBa_K299802 DNA Aactag - like a biobrick scar but first T changed to A
 
BBa_K299802 DNA Aactag - like a biobrick scar but first T changed to A
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BBa_K299803 DNA Gactag - like a biobrick scar but first T changed to G
 
BBa_K299803 DNA Gactag - like a biobrick scar but first T changed to G
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BBa_K299804 DNA Cactag - like a biobrick scar but first T changed to C.
 
BBa_K299804 DNA Cactag - like a biobrick scar but first T changed to C.
  

Revision as of 23:28, 16 October 2010

RBS measurement device pT7 J61107 GFP

This part contains RBS J61107 from the Anderson's collection.

In the physical DNA after J61107 in composite parts there is Cactag sequence instead of the biobrick scar Tactag. J61101 was originally designed in a way that its last nucleotide "C" is also first nucleotide of the suffix (wchich should be T)- it modifies the suffix. The sequence of J61107 in the registry does not include its last nucleotide. If it were included the registry sequence would show the additional nucleotide, now it shows T instead of C.

The seguence of the J612107 does not match its physical DNA

To go around this problem in out bricks we used scarless assembly option and manually included the scars:

BBa_K299800 DNA standard biobrick scar (if next part starts with ATG)

BBa_K299801 DNA standard biobrick scar (if next sequence does NOT start with ATG)

BBa_K299802 DNA Aactag - like a biobrick scar but first T changed to A

BBa_K299803 DNA Gactag - like a biobrick scar but first T changed to G

BBa_K299804 DNA Cactag - like a biobrick scar but first T changed to C.

This brick consists of the following subparts:

I719005[T7promoter] BBa_K299801[standard scar] BBa_J61107[RBS] BBa_K299804[Cactag] BBa_E0040[GFP] BBa_K299801[standard scar] BBa_B0010[terminator] BBa_K299801[standard scar] BBa_B0012[terminator]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 693