Difference between revisions of "Part:BBa K424018"

Revision as of 20:01, 15 October 2010

Rhamnosiltransferase BioBrick (Rh1AB_BB)

Pseudomonas aeruginosa species of bacteria produce the rhamnosyltransferase gene complex (RhlAB) that is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid. How to used it? We developed this standarized part to be inserted into E. coli for rhamnolipid production through the key enzyme rhamnosyltranferase. For this we provide to E. coli the proper sustrate to rhamnosiltransferase biosinthesize rhamnolipids in vitro.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 169
Illegal BamHI site found at 729
Illegal XhoI site found at 905
Illegal XhoI site found at 2191
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1084
Illegal NgoMIV site found at 1805
Illegal NgoMIV site found at 1918
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 394
Illegal BsaI site found at 1434
Illegal BsaI.rc site found at 578

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K424018 short</partinfo>
-Pseudomonas aeruginosa species of bacteria produce the rhamnosyltransferase 1 complex (RhlAB) that is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid.
+Pseudomonas aeruginosa species of bacteria produce the rhamnosyltransferase gene complex (RhlAB) that is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid.
-How to used it??
+How to used it?
 We developed this standarized part to be inserted into E. coli for rhamnolipid production through the key enzyme rhamnosyltranferase. For this we provide to E. coli the proper sustrate to rhamnosiltransferase biosinthesize rhamnolipids in vitro.