Difference between revisions of "Part:BBa J18932"
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* Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli | * Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli | ||
| − | * Contains a hidden translation start site in the N-terminal -> up to 50% of protein produced in E.coli will be truncated | + | * Contains a hidden translation start site in the N-terminal -> up to 50% of protein produced in E.coli will be truncated (R. Grünberg, unpublished) |
* SDS treatment and boiling before PAGE hydrolyzes the chromophore at F//MYG splitting the protein in half (discussed in Gross et al. (2000) The structure of the chromophore within DsRed protein from coral.) | * SDS treatment and boiling before PAGE hydrolyzes the chromophore at F//MYG splitting the protein in half (discussed in Gross et al. (2000) The structure of the chromophore within DsRed protein from coral.) | ||
* Best maturation for expression at 20 or 25 C (rather than 37) | * Best maturation for expression at 20 or 25 C (rather than 37) | ||
Revision as of 17:17, 14 October 2010
mCherry RFP
Red fluorescent protein derived from DsRed.
Advantages:
- fast folding and maturation
- bright and photo-stable
Purity issues (update):
- Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli
- Contains a hidden translation start site in the N-terminal -> up to 50% of protein produced in E.coli will be truncated (R. Grünberg, unpublished)
- SDS treatment and boiling before PAGE hydrolyzes the chromophore at F//MYG splitting the protein in half (discussed in Gross et al. (2000) The structure of the chromophore within DsRed protein from coral.)
- Best maturation for expression at 20 or 25 C (rather than 37)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]