Difference between revisions of "Part:BBa K404208"
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[[Image:Freiburg10_ViralBrick-logo-587-BLA.png|thumb|center|480px]]<br> | [[Image:Freiburg10_ViralBrick-logo-587-BLA.png|thumb|center|480px]]<br> | ||
− | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | ===Restriction sites=== | ||
+ | |||
+ | <table border="1" style="float:right; width:480px; height:auto;> | ||
+ | <tr> | ||
+ | <th><b>Restriction site</b></th> | ||
+ | <th><b>Enzyme</b></th> | ||
+ | <th><b>Recognition sequence</b></th> | ||
+ | <th><b>Activity</b></th> | ||
+ | <th><b>Temp.</b></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>upstream 453</td> | ||
+ | <td>[http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF]</td> | ||
+ | <td>[[image:Freiburg10_recognition site SspI-HF.Gif]]</td> | ||
+ | <td>25;100;0;100</td> | ||
+ | <td>37°C</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>downstream 453</td> | ||
+ | <td>[http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF]</td> | ||
+ | <td>[[image:Freiburg10_recognition site SalI-HF.Gif]]</td> | ||
+ | <td>10;100;100;100</td> | ||
+ | <td>37°C</td> | ||
+ | |||
+ | <tr> | ||
+ | </table> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 15:49, 26 October 2010
ViralBrick-587-Beta Lactamase (BLA)
Usage and Biology
Restriction sites
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]