Difference between revisions of "Part:BBa K314103"
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<partinfo>BBa_K314103 short</partinfo> | <partinfo>BBa_K314103 short</partinfo> | ||
| − | Lac induced protein expression insert includes f1 origin, Lac I | + | Lac induced protein expression insert includes f1 origin K314110, Lac I generator K314111, a lac I repressed promoter R0011, and the Elowitz standard RBS B0034. When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 02:04, 21 October 2010
Lac induced expression cassette
Lac induced protein expression insert includes f1 origin K314110, Lac I generator K314111, a lac I repressed promoter R0011, and the Elowitz standard RBS B0034. When ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]