Difference between revisions of "Part:BBa K314100"
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<partinfo>BBa_K314100 short</partinfo> | <partinfo>BBa_K314100 short</partinfo> | ||
| − | High constitutive protein expression insert includes f1 origin and RBS B0034 | + | High constitutive protein expression insert includes f1 origin k314110, a high expression promoter J23100, and the standard Elowitz standard RBS B0034. When digested and ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression. |
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Revision as of 01:52, 21 October 2010
High constitutive expression cassette
High constitutive protein expression insert includes f1 origin k314110, a high expression promoter J23100, and the standard Elowitz standard RBS B0034. When digested and ligated with a protein coding sequence at the SpeI site the scar site created will create proper spacing for protein expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]