Difference between revisions of "Part:BBa K322211:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, which were presumably present in BBa_I712019 to make a proper eukaryotic context for the start codon, were removed for improved expression in ''E. coli''.  
 
This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, which were presumably present in BBa_I712019 to make a proper eukaryotic context for the start codon, were removed for improved expression in ''E. coli''.  
  
−
Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.
+
Note that the luciferase is temperature sensitive. Growing the chassis at 30°C will result in considerably increased brightness.
  
 
===Source===
 
===Source===

Latest revision as of 17:54, 25 October 2010

Red emitting firefly luciferase mutant (356K)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808


Design Notes

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, which were presumably present in BBa_I712019 to make a proper eukaryotic context for the start codon, were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the chassis at 30°C will result in considerably increased brightness.

Source

Photinus pyralis, from BBa_I712019

References

Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007).