Difference between revisions of "Part:BBa K389011:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
  
−
synthetic ''virG'' gene, ''vir'' promotor from ''A. tumefaciens'' C58, parts.igem
+
* ''virG'' gene synthesized by Mr. Gene (<partinfo>K389002</partinfo>)
 +
* ''vir'' promoter from ''A. tumefaciens'' C58 (partinfo>K389003</partinfo>)
 +
* constitutive promoter and double terminator from parts.igem (<partinfo>J23110</partinfo>, <partinfo>B0017</partinfo>)
  
 
===References===
 
===References===

Revision as of 12:33, 7 October 2010

VirA screening device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • medium strong constitutive promoter,
  • double terminator (forward) to keep basal expression of kanamycin resistance low
  • kanamycin resistance for screening with plates

Source

  • virG gene synthesized by Mr. Gene (BBa_K389002)
  • vir promoter from A. tumefaciens C58 (partinfo>K389003</partinfo>)
  • constitutive promoter and double terminator from parts.igem (BBa_J23110, BBa_B0017)

References