Difference between revisions of "Part:BBa K395702:Design"
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+ | Tomoko Nishizaki et.al Metabolic Engineering of Carotenoid Biosynthesis in Escherichia coli | ||
+ | by Ordered Gene Assembly in Bacillus subtilis�Appl Environ Microbiol v.73(4); Feb 2007 |
Revision as of 10:35, 21 October 2010
rbs+crtZ under pBad promoter for double plasmids
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
・This operon is under arabinose inducible promoter.
・I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmids in the project.
Source
Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.
This Composite Biobrick is created by standard assembly of Part BBa_I0500, Part BBa_I742158.
References
Tomoko Nishizaki et.al Metabolic Engineering of Carotenoid Biosynthesis in Escherichia coli by Ordered Gene Assembly in Bacillus subtilis�Appl Environ Microbiol v.73(4); Feb 2007