Difference between revisions of "Part:BBa K395704:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K395704 short</partinfo>
 
<partinfo>BBa_K395704 short</partinfo>
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&#12539;crtZ is under Arabinose inducible promoter.  
 
&#12539;crtZ is under Arabinose inducible promoter.  
  
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&#12539;crtZ is located at the upper of crtEBIY because the suffix of BBa_K274210(crtEBIY) is out of order and it is impossible to bring it at the lower.
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&#12539;crtZ is located at upstream of crtEBIY because the suffix of BBa_K274210(crtEBIY) is out of order and it is impossible to bring it at downstream.
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===Source===
 
===Source===

Revision as of 11:12, 21 October 2010

zeaxanthin synthesis operon (pBad+rbs+crtZ+plac+rbs +crtEBIY )


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3810
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3346
    Illegal NgoMIV site found at 3476
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

・crtZ is under Arabinose inducible promoter.

・crtZ is located at upstream of crtEBIY because the suffix of BBa_K274210(crtEBIY) is out of order and it is impossible to bring it at downstream.

Source

Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae.

This Composite Biobrick is created by standard assembly of Part BBa_K395702, Part BBa_K274210.



References