Difference between revisions of "Part:BBa K389014"

Revision as of 17:03, 27 October 2010

VirA/G screening system test

This BioBrick is made to test our virA screening system and the strategy for directed evolution, respectively. It contains an unmutated virA under the control of a constitutive promoter and the screening device BBa_K389011.

Usage and Biology

This Device can be used for characterization and screening of mutated VirA receptors.

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389015

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1632
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1768

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K389014 short</partinfo>
@@ Line 5: / Line 4: @@
 This BioBrick is made to test our ''virA'' screening system and the strategy for directed evolution, respectively. It contains an unmutated ''virA'' under the control of a constitutive promoter and the screening device <partinfo>K389011</partinfo>.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This Device can be used for characterization and screening of mutated VirA receptors.
+For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389015</partinfo>
 <!-- -->