Difference between revisions of "Part:BBa K318502"

 
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<partinfo>BBa_K318502 short</partinfo>
 
<partinfo>BBa_K318502 short</partinfo>
  
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Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices:BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB)
  
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===Usage and Biology===
 
===Usage and Biology===
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RcsA and RcsB  are transcription factors that are positive regulator of capsular polysaccharide synthesis. The two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. RcsB is also know to form a homodimmer and positivly regulate cell division. RcsA and RcsB belong to the multicomponent RcsF/RcsC
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See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA]]
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See more on [[http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10821 Ecocyc-RcsB]]
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===Characterization===
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Zip File Download
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(Testing Data Here)
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K318502 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K318502 SequenceAndFeatures</partinfo>

Revision as of 23:03, 8 October 2010

lacI pL + RBS + RcsB + RBS + RcsA + TT

Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices:BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB)

Usage and Biology

RcsA and RcsB are transcription factors that are positive regulator of capsular polysaccharide synthesis. The two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. RcsB is also know to form a homodimmer and positivly regulate cell division. RcsA and RcsB belong to the multicomponent RcsF/RcsC

See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10821 Ecocyc-RcsB

Characterization

Zip File Download

(Testing Data Here)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]