Difference between revisions of "Part:BBa K385004:Experience"
I.stansfield (Talk | contribs) (→Applications of BBa_K385004) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
| Line 5: | Line 4: | ||
===Applications of BBa_K385004=== | ===Applications of BBa_K385004=== | ||
| + | The N-peptide reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast ''Saccharomyces cerevisiae'' in the single copy shuttle vector pRS415. | ||
| + | |||
| + | The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed using a fluorescence microscope optimised for GFP visualisation (Figure 1). | ||
| + | |||
| + | [[Image:N25 + Gal-3.jpg|center|400 px]] | ||
| + | |||
| + | A control culture of the same transformant was grown using glucose as the carbon source; these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence. | ||
| + | |||
| + | Overall the results indicate that the N-peptide can be successfully expressed as a protein fusion with other standard parts. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 12:03, 27 September 2010
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K385004
The N-peptide reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast Saccharomyces cerevisiae in the single copy shuttle vector pRS415.
The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed using a fluorescence microscope optimised for GFP visualisation (Figure 1).
A control culture of the same transformant was grown using glucose as the carbon source; these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence.
Overall the results indicate that the N-peptide can be successfully expressed as a protein fusion with other standard parts.
User Reviews
UNIQ86ef3e8ac121705a-partinfo-00000000-QINU UNIQ86ef3e8ac121705a-partinfo-00000001-QINU