Difference between revisions of "Part:BBa K322123"
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| − | Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor. It is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon. | + | Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor. It is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon. The there is a weak RBS (B0032) before the heme oxygenase gene and a strong RBS (B0034) before the pcyA gene. |
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Latest revision as of 09:49, 18 August 2011
Phycobilin synthesis operon without terminator
Phycobilin is chromophores (light-capturing molecules) found in cyanobacteria and in the chloroplasts of red algae, glaucophytes and some cryptomonads (though not in green algae and higher plants)， used in light sensor. It is derived from K098010 but PCR was used to remove the terminator, so that cph8 could be added at the end of the operon. The there is a weak RBS (B0032) before the heme oxygenase gene and a strong RBS (B0034) before the pcyA gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
- 1000COMPATIBLE WITH RFC[1000]