Difference between revisions of "Part:BBa K322246"

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<partinfo>BBa_K322246 short</partinfo>
 
<partinfo>BBa_K322246 short</partinfo>
  
This is the same as the other BioBrick BBa-K322211 but mutation S284T was introduced, which has been reported to result in a red-shifted output.
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Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter.  
  
Features: S284T mutation 850 to 852
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In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light
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This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.
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Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.
  
reference:Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007)
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:19, 7 October 2010

Red emitting firefly luciferase mutant (S284T)

Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter.

In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 808