Difference between revisions of "Part:BBa K322246"

Revision as of 19:19, 7 October 2010

Red emitting firefly luciferase mutant (S284T)

Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter.

In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light

This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.

Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 808

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K322246 short</partinfo>
-This is the same as the other BioBrick BBa-K322211 but mutation S284T was introduced, which has been reported to result in a red-shifted output.
+Like BBa_K322211, this luciferase is mutated to have a red shifted output. However it is a lot brighter.
-Features: S284T mutation 850 to 852
+In luminescent reactions, light is produced by the oxidation of a luciferin: luciferin + O2 → oxyluciferin + light
+This biobrick was made from BBa_I712019. 6 bases upstream of the start codon, present in BBa_I712019 (presumably to make a proper eukaryotic context for the start codon), were removed for improved expression in E. coli.
+Note that the luciferase is temperature sensitive. Growing the host at 30°C will result in considerably increased brightness.
-reference:Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366, 131-136. Fujii, H., Noda, K., Asami, Y., Kuroda, A., Sakata, M. & Tokida, A. (2007)
 <!-- Add more about the biology of this part here