Difference between revisions of "Part:BBa K322211:Design"
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===Source=== | ===Source=== | ||
| − | Photinus pyralis | + | Photinus pyralis, from BBa_I712019 |
===References=== | ===References=== | ||
Revision as of 18:47, 7 October 2010
Red emitting firefly luciferase mutant (356K)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 808
Design Notes
Sequence date: the mutation was initially introduced into our construct K216015 and the sequence across the mutation was obtained on 12 Aug 2010 (clone 4). The coding sequence was then amplified by PCR and cloned in pSB1C3 by Will and Lu.
Source
Photinus pyralis, from BBa_I712019