Difference between revisions of "Part:BBa K385003:Experience"

Revision as of 12:04, 27 September 2010

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Applications of BBa_K385003

User Reviews

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 ===Applications of BBa_K385003===
-The N-peptide reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast ''Saccharomyces cerevisiae'' in the single copy shuttle vector pRS415.
-The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed using a fluorescence microscope optimised for GFP visualisation (Figure 1).
-[[Image:N25 + Gal-3.jpg|center|400 px]]
-A control culture of the same transformant was grown using glucose as the carbon source;  these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence.
-Overall the results indicate that the N-peptide can be successfully expressed as a protein fusion with other standard parts.
 ===User Reviews===