Difference between revisions of "Part:BBa K325219"
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This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. | This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. | ||
D-Luciferin has to be added to obtain light output. | D-Luciferin has to be added to obtain light output. | ||
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− | + | ===Usage and Biology=== | |
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− | + | <span class='h3bb'>Sequence and Features</span> | |
− | + | <partinfo>BBa_K325219 SequenceAndFeatures</partinfo> | |
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− | + | <!-- Uncomment this to enable Functional Parameter display | |
− | + | ===Functional Parameters=== | |
− | + | <partinfo>BBa_K325219 parameters</partinfo> | |
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Revision as of 10:48, 24 September 2010
Red Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)
This part generates a red-mutant of the luciferase from the Japanese firefly (L.cruciata) as well as the luciferin regenerating enzyme (LRE). It is under the control of an Arabinose induced promoter. D-Luciferin has to be added to obtain light output.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 3278 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 2169 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 2118
Illegal AgeI site found at 3820 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 1550
Illegal SapI.rc site found at 3334