Difference between revisions of "Part:pSB1A10"

Revision as of 07:49, 26 May 2006

Screening Plasmid v1.0

This plasmid can be used to do a rough characterization of the Input/Output curve for a PoPS-based device. Additionally, it can be used for screening of part or device libaries via FACS. More information can be [http://openwetware.org/index.php?title=Endy:Screening_plasmid_1.0 found here.]

Usage and Biology

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5242
Illegal NheI site found at 4443
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 5248
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5242
Illegal BglII site found at 89
Illegal BamHI site found at 4382
Illegal XhoI site found at 67
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 5242
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 5242
Plasmid lacks a suffix.
Illegal XbaI site found at 5257
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 674
Illegal AgeI site found at 786
Illegal AgeI site found at 4217
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2197
Illegal BsaI.rc site found at 5118
Illegal SapI site found at 4199

Functional Parameters

chassis	-NA-
copies	100-300
mcs	BioBrick
origin	pMB1
resistance	A

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>pSB1A10 short</partinfo>
-This plasmid can be used to do a rough characterization of the Input/Output curve for a PoPS-based device.  More information can be found here:
+This plasmid can be used to do a rough characterization of the Input/Output curve for a PoPS-based device.  Additionally, it can be used for screening of part or device libaries via FACS.  More information can be [http://openwetware.org/index.php?title=Endy:Screening_plasmid_1.0 found here.]
-http://openwetware.org/index.php?title=Endy:Screening_plasmid_1.0