Difference between revisions of "Part:BBa K385002:Experience"

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The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed usin a fluorescence microscope optimised for GFP visualisation (Figure 1).
 
The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed usin a fluorescence microscope optimised for GFP visualisation (Figure 1).
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[[Image:N25 + Gal-3.tif|center|400 px]]
  
  
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Overall the results indicate that the MS2 coat protein can be successfully expressed as a protein fusion with other standard parts.
 
Overall the results indicate that the MS2 coat protein can be successfully expressed as a protein fusion with other standard parts.
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[[Media:N25 + Gal-3.tif]]
 
  
 
===User Reviews===
 
===User Reviews===

Revision as of 16:29, 22 September 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K385002

The MS2 coat protein reading frame was fused in-frame to GFP to make a translational fusion. It was placed under control of the yeast GAL1 promoter (BBa_J63006), and transformed into yeast in the single copy shuttle vector pRS415.

The transformants were grown overnight in synthetic defined medium containing 2% w/v galactose, and observed usin a fluorescence microscope optimised for GFP visualisation (Figure 1). File:N25 + Gal-3.tif


A control culture of the same transformant was grown using glucose as the carbon source; these conditions do not activate the GAL promoter. The results (Figure 2) show no GFP fluorescence.

Overall the results indicate that the MS2 coat protein can be successfully expressed as a protein fusion with other standard parts.

User Reviews

UNIQ4dcd03006db15cea-partinfo-00000000-QINU UNIQ4dcd03006db15cea-partinfo-00000001-QINU