Difference between revisions of "Part:BBa K365002"

 
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<partinfo>BBa_K365002 short</partinfo>
 
<partinfo>BBa_K365002 short</partinfo>
  
 
This part consists in the first 908 amino-acids of Phytochrome B of A. thaliana. When bound to its natural chromophore Phycocyanobiline (PCB) it can reach its Pfr activated state and physically interact with different PIF.
 
This part consists in the first 908 amino-acids of Phytochrome B of A. thaliana. When bound to its natural chromophore Phycocyanobiline (PCB) it can reach its Pfr activated state and physically interact with different PIF.
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<!-- Add more about the biology of this part here
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<p><b>Background:</b></p>
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===Usage and Biology===
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Phytochromes characterised by a red/far-red photochromicity. Through red-light (650–670 nm) absorption the phytochrome undergoes a rapid conformational change from its ground state Pr to its active state Pfr. The structural change allows the binding of the PIF. This light-sensitive interaction has been mapped to the 650-residue amino-terminal photosensory core of PhyB <i><a href="http://2010.igem.org/Team:ESBS-Strasbourg/Project/Reference">(Khanna et al., 2004)</a></i>. The process is completely reversible through absorption in the near infra-red spectrum (705–740 nm).
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<br><br>
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The photoreceptor protein PhyB serves for the light-dependent activation of the system, therefore it will be fused to the N-teminal of the ClpX-trimer.
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<img src="https://static.igem.org/mediawiki/2010/e/e6/ESBS-Strasbourg-PhyB900-black.png" width="250px" height="167px" border="0">
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<a target="_blank" href="http://2010.igem.org/Team:ESBS-Strasbourg/Project/visual"><font>You can visit our wiki "visual description" page for more info</font></a>
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</center>
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<br><br>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
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<p><b>Conception:</b></p>
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In in-vivo applications it has been shown that the PIF-interaction with the PhyB photosensory core (residues 1–650) is irreversible in infrared light. <i><a href="http://2010.igem.org/Team:ESBS-Strasbourg/Project/Reference">Lim & Voigt (2009)</a></i> demonstrated by assaying PIF6 (which has the strongest interactions of all previously reported PIF domains) against different variants of PhyB that the tandem C-terminal PAS domains (residues 1-908)of plant phytochromes are necessary to confer rapid photoreversibility under infrared light.
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The original sequence contains a SpeI restriction within the first 908 residues.
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<partinfo>BBa_K365002 parameters</partinfo>
 
<partinfo>BBa_K365002 parameters</partinfo>
 
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Revision as of 22:03, 26 October 2010

Phytochrome B from A. thaliana (aa 1-908)

This part consists in the first 908 amino-acids of Phytochrome B of A. thaliana. When bound to its natural chromophore Phycocyanobiline (PCB) it can reach its Pfr activated state and physically interact with different PIF.

Background:

Phytochromes characterised by a red/far-red photochromicity. Through red-light (650–670 nm) absorption the phytochrome undergoes a rapid conformational change from its ground state Pr to its active state Pfr. The structural change allows the binding of the PIF. This light-sensitive interaction has been mapped to the 650-residue amino-terminal photosensory core of PhyB (Khanna et al., 2004). The process is completely reversible through absorption in the near infra-red spectrum (705–740 nm).

The photoreceptor protein PhyB serves for the light-dependent activation of the system, therefore it will be fused to the N-teminal of the ClpX-trimer.

You can visit our wiki "visual description" page for more info


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 490
    Illegal BamHI site found at 572
    Illegal XhoI site found at 523
    Illegal XhoI site found at 542
    Illegal XhoI site found at 2677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2062
    Illegal BsaI site found at 2468
    Illegal SapI site found at 739


Conception:

In in-vivo applications it has been shown that the PIF-interaction with the PhyB photosensory core (residues 1–650) is irreversible in infrared light. Lim & Voigt (2009) demonstrated by assaying PIF6 (which has the strongest interactions of all previously reported PIF domains) against different variants of PhyB that the tandem C-terminal PAS domains (residues 1-908)of plant phytochromes are necessary to confer rapid photoreversibility under infrared light. The original sequence contains a SpeI restriction within the first 908 residues.