Difference between revisions of "Part:BBa K404112:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The cytosine deaminase was PCR amplified using the <i>codA</i> gene as PCR template. Additionally, two site-directed mutagenesis had to be performed in order to delete PsI and NgoMIV. | |
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===Source=== | ===Source=== | ||
| − | + | <i>codA</i> | |
===References=== | ===References=== | ||
Latest revision as of 03:25, 25 October 2010
Cytosine deaminase (CD)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cytosine deaminase was PCR amplified using the codA gene as PCR template. Additionally, two site-directed mutagenesis had to be performed in order to delete PsI and NgoMIV.
Source
codA