Difference between revisions of "Part:BBa F2620"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_F2620 short</partinfo>
 
<partinfo>BBa_F2620 short</partinfo>
  
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A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [3OC6HSL] is controlled by an operator [TetR].  Device input is 3OC6HSL.  Device output is PoPS produced at a LuxR-regulated operator.
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This device senses the 3OC<sub>6</sub>HSL concentration in the media and produces output PoPS according to the transfer function given below.  LuxR production is controlled by the tet repressible R0040.  Initial characterization work has been done on this device using I13273 as a test device.
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===Usage and Biology===
 
===Usage and Biology===
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Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section).  If used in a cell containing TetR then a second input signal [aTc] can be used to produce a logical AND function.  
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&lt;img src&#61;"'''Include File F2620Data'''"
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The transfer function indicates a switch point of approximately 2nM which was constant in repeated experiments (data not shown).
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The latency shows that fluorescent proteins appear within moments of the addition of AHL to the media.  This suggests that PoPS output adjusts to a change in input very rapidly.  This is expected given the current model of device operation.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_F2620 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_F2620 SequenceAndFeatures</partinfo>
  
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===Device Characteristics===
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
 
<partinfo>BBa_F2620 parameters</partinfo>
 
<partinfo>BBa_F2620 parameters</partinfo>
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Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]
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====Transfer Function====
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====Latency====
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====Specificity====
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[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]
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====Stability====
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[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]
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Revision as of 02:13, 5 June 2006


3OC6HSL -> PoPS Receiver

This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below. LuxR production is controlled by the tet repressible R0040. Initial characterization work has been done on this device using I13273 as a test device.


Usage and Biology

<img src="Include File F2620Data"

The transfer function indicates a switch point of approximately 2nM which was constant in repeated experiments (data not shown).

The latency shows that fluorescent proteins appear within moments of the addition of AHL to the media. This suggests that PoPS output adjusts to a change in input very rapidly. This is expected given the current model of device operation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004