Difference between revisions of "Part:BBa F2620"

Revision as of 21:04, 14 May 2006

3OC₆HSL -> PoPS Receiver

A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [3OC6HSL] is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.

Usage and Biology

Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal [aTc] can be used to produce a logical AND function.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1004

Device Characteristics

control	R0040
device_type	Receiver
family	-NA-
latency	Seconds
proteins	LuxR, TetR
signalling_molecule	3OC₆HSL
switch_point	2nM

Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]

Transfer Function

Latency

Specificity

[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]

Stability

[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_F2620 short</partinfo>
-This device senses the 3OC<sub>6</sub>HSL concentration in the media and produces output PoPS according to the transfer function given below.  LuxR production is controlled by the tet repressible R0040.  Initial characterization work has been done on this device using T9002 as a test device.
+A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule [3OC6HSL] is controlled by an operator [TetR].  Device input is 3OC6HSL.  Device output is PoPS produced at a LuxR-regulated operator.
 ===Usage and Biology===
-<img src="'''Include File F2620Data'''"
+Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., pSB1A2] results in a reduced cell growth rate (see Load section).  If used in a cell containing TetR then a second input signal [aTc] can be used to produce a logical AND function.
-The transfer function indicates a switch point of approximately 2nM which was constant in repeated experiments (data not shown).
-The latency shows that fluorescent proteins appear within moments of the addition of AHL to the media.  This suggests that PoPS output adjusts to a change in input very rapidly.  This is expected given the current model of device operation.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_F2620 SequenceAndFeatures</partinfo>
-===Functional Parameters===
+===Device Characteristics===
 <partinfo>BBa_F2620 parameters</partinfo>
 Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]
+====Transfer Function====
+====Latency====
+====Specificity====
+[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]
+====Stability====
+[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]