Difference between revisions of "Part:BBa K389010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The ''virA'' gene is under the control a medium strong constitutive | + | The ''virA'' gene is under the control a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein) |
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===Source=== | ===Source=== | ||
Revision as of 12:01, 7 October 2010
VirA receptor (expressed)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768
Design Notes
The virA gene is under the control a medium strong constitutive promoter so there is enough VirA receptor expressed but not too much so the cell suffers from the expression (VirA is a membrane protein)
Source
A. tumefaciens C58, parts.igem