Difference between revisions of "Part:BBa K389002"

Revision as of 11:42, 4 October 2010

Mutated virG

This is the virG response regulator from the VirA/G receptor system. The virA/G receptor system is used by Agrobacterium tumefaciens to detect phenolic substances secreted by injured plants, e.g. acetosyringone. In presence of these substances, VirG is activated by the VirA receptor and induces the transcription of genes under the control of a vir promoter. Normally, VirG needs the rpoA RNA polymerase subunit from A. tumefaciens to work in Escherichia coli but this virG BioBrick is mutated, so it works with the rpoA subunit from E. coli. For this reason the point mutations G56V and I77V were brought into the gene (compare YC Jung et al., 2004). Because this BioBrick is synthesized (Mr.Gene GmbH), codon usage is optimized for E. coli and illegal restriction sites were removed. When you use this virG gene in a VirA/G signaling system you do not need BBa_K238010 anymore to get the system working in E. coli.

Usage and Biology

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001 and a vir promoter BBa_K389003. You can find an already assembled VirA/G-receptor system here: BBa_K389017.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K389002 short</partinfo>
-This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
+This is the ''virG'' response regulator from the VirA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by injured plants, ''e.g.'' acetosyringone. In presence of these substances, VirG is activated by the VirA receptor and induces the transcription of genes under the control of a ''vir'' promoter. Normally, VirG needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so it works with the ''rpoA'' subunit from ''E. coli''. For this reason the point mutations G56V and I77V were brought into the gene (compare YC Jung ''et al.'', 2004). Because this BioBrick is synthesized (Mr.Gene GmbH), codon usage is optimized for ''E. coli'' and illegal restriction sites were removed. When you use this ''virG'' gene in a VirA/G signaling system you do not need <partinfo>BBa_K238010</partinfo> anymore to get the system working in ''E. coli''.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>BBa_K389001</partinfo> and a ''vir'' promoter <partinfo>BBa_K389003</partinfo>. You can find an already assembled VirA/G-receptor system here: <partinfo>BBa_K389017</partinfo>.
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