Difference between revisions of "Part:BBa C0012"
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<partinfo>BBa_C0012 short</partinfo>
 
<partinfo>BBa_C0012 short</partinfo>
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A rapid degredation tail (LVA) has been added to improve the High to Low      performance of this part.
 
A rapid degredation tail (LVA) has been added to improve the High to Low      performance of this part.
  
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[[Image:LacRep-black.jpg]]
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_C0012 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_C0012 parameters</partinfo>
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Revision as of 01:22, 5 June 2006


lacI repressor from E. coli (+LVA)

Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.

A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1147
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]