Difference between revisions of "Part:BBa J72016:Design"
Jennbrophy (Talk | contribs) (→Design Notes) |
Jennbrophy (Talk | contribs) (→Wobble construction of Bmll56 {rbs_at_10000.6XHis>}) |
||
| Line 24: | Line 24: | ||
CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG | CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG | ||
| − | + | </pre> | |
===References=== | ===References=== | ||
Latest revision as of 21:07, 15 July 2010
{Bmll56} in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
Overlap Extension PCR (Wobble construction)
Wobble construction of Bmll56 {rbs_at_10000.6XHis>}
Wobble mll56/mll57 (88, EcoRI/BamHI)
Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L)
Product is pBca9523-Bmll56 {rbs_at_10000.6XHis>}
----
mll56 Forward wobble for {rbs_at_10000.6XHis>} (Bmll56)
CCATAGAATTCATGAGATCTCTACAACACCTAGCATCTAAGAAGTTACATATGGATTC
mll57 Reverse wobble for {rbs_at_10000.6XHis>} (Bmll56)
CGTTAGGATCCGTGATGGTGATGATGGTGAGAATCCATATGTAACTTCTTAG