Difference between revisions of "Part:BBa I739001:Experience"

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I used this part for constitutive expression of tetR. After transforming the device DNA from the 2010 iGEM distribution into DH5a E.coli, I miniprepped the sample, cut with EcoRI and PstI, and ran on a gel. The insert was ~2400bp, and the plasmid was ~3000bp. This is quite a bit more than the ~900bp the part is supposed to be. I decided to instead use Q04400 and add a constitutive promoter in front. I did not test to see whether I739001 still expressed tetR.   
 
I used this part for constitutive expression of tetR. After transforming the device DNA from the 2010 iGEM distribution into DH5a E.coli, I miniprepped the sample, cut with EcoRI and PstI, and ran on a gel. The insert was ~2400bp, and the plasmid was ~3000bp. This is quite a bit more than the ~900bp the part is supposed to be. I decided to instead use Q04400 and add a constitutive promoter in front. I did not test to see whether I739001 still expressed tetR.   
 
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Latest revision as of 22:23, 7 July 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I739001

User Reviews

UNIQ276c9b9e8910074c-partinfo-00000000-QINU UNIQ276c9b9e8910074c-partinfo-00000001-QINU

•

Michael Pettigrew

I used this part for constitutive expression of tetR. After transforming the device DNA from the 2010 iGEM distribution into DH5a E.coli, I miniprepped the sample, cut with EcoRI and PstI, and ran on a gel. The insert was ~2400bp, and the plasmid was ~3000bp. This is quite a bit more than the ~900bp the part is supposed to be. I decided to instead use Q04400 and add a constitutive promoter in front. I did not test to see whether I739001 still expressed tetR.