Difference between revisions of "Part:BBa K416003"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K416003 short</partinfo>
 
<partinfo>BBa_K416003 short</partinfo>
  
−
This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein.  After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag.  To use this tag, attached it directly upstream of your proteins CDS.  
+
This secretion tag can direct extracellular secretion of any protein fused to it when linked to the protein's C-terminus of that protein.  After targeting the fusion through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease in the trans golgi, resulting in secretion of protein without this tag attached.  To use this tag, attached it directly to your proteins CDS.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:19, 28 October 2010

Yeast Secretion Tag

This secretion tag can direct extracellular secretion of any protein fused to it when linked to the protein's C-terminus of that protein. After targeting the fusion through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease in the trans golgi, resulting in secretion of protein without this tag attached. To use this tag, attached it directly to your proteins CDS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]