Difference between revisions of "Part:BBa J70565:Design"

(→‎Design Notes)
(→‎Design Notes)
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A 10 amino Gly-Ser linker was chosen to maintain functionality of the dimer, but this was fairly arbitrary and the length of the intervening linker may need to be changed.
 
A 10 amino Gly-Ser linker was chosen to maintain functionality of the dimer, but this was fairly arbitrary and the length of the intervening linker may need to be changed.
  
−
Also there appears to be a mutation
+
Additionally, a missence mutation occurred during amplification of K216008, in which nucleotide 1393 and 1394 (numbers refer to part BBaJ70565's sequence) were switched causing the sequence to read cctgtcc'''gc'''ata instead of cctgtcc'''cg'''ata
  
 
===Source===
 
===Source===

Revision as of 19:14, 30 March 2010

luxAB Fusion gene, Xenorhabdus luminescens


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1049


Design Notes

A 10 amino Gly-Ser linker was chosen to maintain functionality of the dimer, but this was fairly arbitrary and the length of the intervening linker may need to be changed.

Additionally, a missence mutation occurred during amplification of K216008, in which nucleotide 1393 and 1394 (numbers refer to part BBaJ70565's sequence) were switched causing the sequence to read cctgtccgcata instead of cctgtcccgata

Source

Amplified from part K216008.

References