Difference between revisions of "Part:BBa V1005"
 
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<partinfo>BBa_V1005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_V1005 SequenceAndFeatures</partinfo>
  
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==References==
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===References===
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*"Positive-selection vectors using the F plasmid <i>ccdB</i> killer gene." Bernard P, Gabant P, Bahassi EM, Couturier M. 1994 Gene. Oct 11;148(1):71-4. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]
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*Bernard P, Gabant P, Bahassi EM, Couturier M, Université Libre de Bruxelles. <i>Positive-selection vectors using the F plasmid </i>ccdB<i> killer gene.</i>" Gene 1994 Oct 11;148(1):71-4. pmid:7926841. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]
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*Bernard P, Gabant P, Université Libre de Bruxelles. <i>Cloning and/or sequencing vector</i> US Patent Number 5,910,438, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
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*Bernard P, Gabant P, Université Libre de Bruxelles. <i>Cloning and/or sequencing vector</i> US Patent Number 6,180,407 B1, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
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*Bernard P, Gabant P, Université Libre de Bruxelles. <i>Cloning and/or sequencing vector</i> US Patent Number 7,176,029 B2, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]
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* Bahassi, et al., Université Libre de Bruxelles. <i>Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA.</i>, J. Biol. Chem. 1999 274: 10936-10944. pmid:10196173. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]
  
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*US Patent Number 5,910,438 "Cloning and/or sequencing vector" Bernard P, Gabant P, University Libre de Bruxelles, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
 
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*US Patent Number 6,180,407 B1 "Cloning and/or sequencing vector" Bernard P, Gabant P, Universit Libre de Bruxelles, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
 
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*US Patent Number 7,176,029 B2 "Cloning and/or sequencing vector"  Bernard P, Gabant P, Universit Libre de Bruxelles, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]
 
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*"Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA." Bahassi, et al. 1999 J. Biol. Chem. 274: 10936-10944 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]
 
 
*[[Help:Plasmids]]
 
*[[Help:Plasmids]]
 
*[[Part:BBa_P1010]]
 
*[[Part:BBa_P1010]]

Latest revision as of 18:00, 16 March 2010

DB3.1

E. coli strain containing the gyrA462 mutation, conveying recessive resistance to the ccdB gene.
Strain created by and available for purchase through Invitrogen


Usage and Biology

  • useful for propagating plasmids containing the ccdB operon.
  • gyrA462 enables ccdB containing plasmid propagation
  • streptomycin resistant


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  • Bernard P, Gabant P, Bahassi EM, Couturier M, Université Libre de Bruxelles. Positive-selection vectors using the F plasmid ccdB killer gene." Gene 1994 Oct 11;148(1):71-4. pmid:7926841. [http://www.ncbi.nlm.nih.gov/pubmed/7926841 Pubmed]
  • Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 5,910,438, 1999. [http://www.google.com/patents?vid=USPAT5910438 Google Patents]
  • Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 6,180,407 B1, 2001. [http://www.google.com/patents?vid=USPAT6180407 Google Patents]
  • Bernard P, Gabant P, Université Libre de Bruxelles. Cloning and/or sequencing vector US Patent Number 7,176,029 B2, 2007. [http://www.google.com/patents?vid=USPAT7176029 Google Patents]
  • Bahassi, et al., Université Libre de Bruxelles. Interactions of CcdB with DNA gyrase. Inactivation of Gyra, poisoning of the gyrase-DNA complex, and the antidote action of CcdA., J. Biol. Chem. 1999 274: 10936-10944. pmid:10196173. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10196173&query_hl=2&itool=pubmed_ExternalLink Pubmed]