Difference between revisions of "Part:BBa J18932"

Revision as of 17:14, 14 October 2010

mCherry RFP

Red fluorescent protein derived from DsRed.

Advantages:

Purity issues (update):

Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli
Contains a hidden translation start site in the N-terminal -> up to 50% of protein produced in E.coli will be truncated
SDS treatment and boiling before PAGE hydrolyzes the chromophore at F//MYG splitting the protein in half
Best maturation for expression at 20 or 25 C (rather than 37)

Sequence and Features

Assembly Compatibility:

@@ Line 9: / Line 9: @@
 * bright and photo-stable
-'''Degradation issues:'''
+'''Purity issues (update):'''
 * Ajo-Franklin...Silver (2007) report multiple bands for mCherry purifications in E. coli
-* SDS treatment or boiling can hydrolyze the chromophore position at F//MYG splitting the protein in half
+* Contains a hidden translation start site in the N-terminal -> up to 50% of protein produced in E.coli will be truncated
+* SDS treatment and boiling before PAGE hydrolyzes the chromophore at F//MYG splitting the protein in half
+* Best maturation for expression at 20 or 25 C (rather than 37)
 <!-- Add more about the biology of this part here