Difference between revisions of "Part:BBa J18911"

Latest revision as of 09:50, 25 January 2010

RFC 25 vector reverse (prefix)

reverse primer for vector linearization of any vector containing the RFC 25 Biobrick flanks, anneals to prefix covering NgoMIV and XbaI site

   * Tm (oligocalc nearest neighbore, 500nM primer, 50mM salt) = 60 C

   * Tm (finnzyme, 500nM primer, 50mM salt) = 68

   * Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 66.5

Revision as of 09:50, 25 January 2010 (view source) Raik (Talk \| contribs) ← Older edit		Latest revision as of 09:50, 25 January 2010 (view source) Raik (Talk \| contribs)
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	* Tm (finnzyme, 500nM primer, 50mM salt) = 68		* Tm (finnzyme, 500nM primer, 50mM salt) = 68
−		+
	* Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 66.5		* Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 66.5