Difference between revisions of "Part:BBa J18910"

Latest revision as of 09:53, 25 January 2010

RFC 25 vector forward (suffix)

forward primer for vector linearization of any vector containing the RFC 25 Biobrick flanks, anneals to suffix covering AgeI and SpeI site

   * Tm (oligocalc nearest neighbore, 500nM primer, 50mM salt) = 60 C
   * Tm (finnzyme, 500nM primer, 50mM salt) = 65
   * Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 67.5

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 __NOTOC__
 <partinfo>BBa_J18910 short</partinfo>
-Primer for backbone linearization of RFC 25 construction vectors. Aligns to the RFC 25 prefix but pointing outward into the backbone rather than insert.
+forward primer for vector linearization of any vector containing the RFC 25 Biobrick flanks, anneals to suffix covering AgeI and SpeI site
+    * Tm (oligocalc nearest neighbore, 500nM primer, 50mM salt) = 60 C
+    * Tm (finnzyme, 500nM primer, 50mM salt) = 65
+    * Tm (biophp, 500mM, 50mM, 1.5mM MgCl2) = 67.5
-Annealing temperature: 68 C (in Phusion polymerase buffer)
+See also:  [[J18911]] for reverse
 <!-- Add more about the biology of this part here