Difference between revisions of "Part:BBa K5380001:Experience"

(→‎Applications of BBa_K5380001)
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014).
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how you used this part and how it worked out.
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===Applications of BBa_K5380001===
 
===Applications of BBa_K5380001===
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We used this part in our project as a promoter to up regulate downstream genes in response to the presence of nitrate in our plant system. We were unable to verify its efficiency in our plant, as we were unable to verify the insertion of the part, but its efficacy is well validated in previous publications.
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Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014). We used this part in our project as a promoter to up regulate downstream genes in response to the presence of nitrate in our plant system. We were unable to verify its efficiency in our plant, as we were unable to verify the insertion of the part, but its efficacy is well validated in previous publications.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 13:47, 2 October 2024


Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014).

Applications of BBa_K5380001

Arabidopsis Thaliana utilizes NIN-Like Proteins (NLPs), which undergo conformational changes in the presence of Nitrate (Konishi et al., 2013; Chardin et al., 2014). NLPs, having gone aforementioned conformations, will interact as transcription factors by binding to Nitrate Responsive Cis-Elements (NREs), thus increasing expression rates of downstream elements (Konishi et al., 2013; Chardin et al., 2014). We used this part in our project as a promoter to up regulate downstream genes in response to the presence of nitrate in our plant system. We were unable to verify its efficiency in our plant, as we were unable to verify the insertion of the part, but its efficacy is well validated in previous publications.

User Reviews

UNIQc9cf7698c3fef91e-partinfo-00000000-QINU UNIQc9cf7698c3fef91e-partinfo-00000001-QINU