Difference between revisions of "Part:BBa K188634"
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So this device only can be used to add a specific functional promoter(and other target gene), and measure the activity of the promoter by measure the expression of mRFP. | So this device only can be used to add a specific functional promoter(and other target gene), and measure the activity of the promoter by measure the expression of mRFP. | ||
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Revision as of 21:41, 31 October 2009
RBS+mRFP+double Terminator
This device is part of device F(BBa_K188661) in our project without ccdB gene(BBa_K145151) and promoter lacI/pL(BBa_R0011). We expect that the AHL-luxR complex would bind to promoter lacI/pL(BBa_R0011) and express the the ccdB gene for E. coli suicide when project is done. And express mRFP to indicate the the project is done.
So this device only can be used to add a specific functional promoter(and other target gene), and measure the activity of the promoter by measure the expression of mRFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 573
Illegal AgeI site found at 685 - 1000COMPATIBLE WITH RFC[1000]