Difference between revisions of "Part:BBa K5127015:Design"
Enolyn1214 (Talk | contribs) |
Enolyn1214 (Talk | contribs) (→Design Notes) |
||
| Line 11: | Line 11: | ||
<HTML> | <HTML> | ||
| − | <p style="text-align:center;"><img src="https://static.igem.wiki/teams/5127/results/ | + | <p style="text-align:center;"><img src="https://static.igem.wiki/teams/5127/results/23.jpg" width="400" height="auto"/> |
<br> | <br> | ||
<i>Figure 1. Plasmid design of pDual-Select. Created by biorender.com.</i> | <i>Figure 1. Plasmid design of pDual-Select. Created by biorender.com.</i> | ||
| Line 17: | Line 17: | ||
</html> | </html> | ||
| − | |||
| − | |||
===Source=== | ===Source=== | ||
Latest revision as of 10:09, 2 October 2024
Device for genome integration (pDual-select)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pDual-select plasmid was assembled with an R6K origin of replication (ori), which can only replicate in E. coli DH5-alpha pir+ strain. This design ensures that when the plasmid is transformed into E. coli MG1655, they cannot replicate, preventing the presence of redundant plasmid copies and avoiding false-positive results during integration verification (Figure 1).
Figure 1. Plasmid design of pDual-Select. Created by biorender.com.
Source
E. Coli MG1655
References
Byung Jo Yu, Kui Hyeon Kang, Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, & Sun Chang Kim. (2008). Rapid and efficient construction of markerless deletions in the Escherichia coli genome. Nucleic Acids Research, 36(14), e84–e84. https://doi.org/10.1093/nar/gkn359