Difference between revisions of "Part:BBa K5127015:Design"
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===Design Notes===
 
===Design Notes===
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The pDual-select plasmid was assembled with an R6K origin of replication (ori), which can only replicate in E. coli DH5-alpha pir+ strain. This design ensures that when the plasmid is transformed into E. coli MG1655, they cannot replicate, preventing the presence of redundant plasmid copies and avoiding false-positive results during integration verification (Figure 1).  
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The pDual-select plasmid was assembled with an R6K origin of replication (ori), which can only replicate in E. coli DH5-alpha pir+ strain. This design ensures that when the plasmid is transformed into <i>E. coli MG1655</i>, they cannot replicate, preventing the presence of redundant plasmid copies and avoiding false-positive results during integration verification (Figure 1).  
  
 
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<p style="text-align:center;"><img src="https://static.igem.wiki/teams/5127/results/21.jpg" width="400" height="auto"/>
 
<p style="text-align:center;"><img src="https://static.igem.wiki/teams/5127/results/21.jpg" width="400" height="auto"/>
 
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</i>Figure 1. Plasmid design of pDual-Select. Created by biorender.com.<i>
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<i>Figure 1. Plasmid design of pDual-Select. Created by biorender.com.</i>
 
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Revision as of 10:06, 2 October 2024


Device for genome integration (pDual-select)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pDual-select plasmid was assembled with an R6K origin of replication (ori), which can only replicate in E. coli DH5-alpha pir+ strain. This design ensures that when the plasmid is transformed into E. coli MG1655, they cannot replicate, preventing the presence of redundant plasmid copies and avoiding false-positive results during integration verification (Figure 1).


Figure 1. Plasmid design of pDual-Select. Created by biorender.com.


Source

E. Coli MG1655


References

Byung Jo Yu, Kui Hyeon Kang, Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, & Sun Chang Kim. (2008). Rapid and efficient construction of markerless deletions in the Escherichia coli genome. Nucleic Acids Research, 36(14), e84–e84. https://doi.org/10.1093/nar/gkn359