Difference between revisions of "Part:BBa K5136234"
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===SD7=== | ===SD7=== | ||
A SD sequence upstream of LexRO encoding gene. SD7 is a ribosome binding site on the genome , which is capable of recruiting ribosomes in engineered <i>E. coli.</i> | A SD sequence upstream of LexRO encoding gene. SD7 is a ribosome binding site on the genome , which is capable of recruiting ribosomes in engineered <i>E. coli.</i> | ||
| − | + | ==<b>Characterization</b>== | |
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Revision as of 08:36, 2 October 2024
J23106-SD7-LexRO-B0015
Biology
LexRO
LexRO is a synthetic light-switchable repressor, based on a novel LOV light sensor domain, RsLOV. In the darkness, LexRO dimerizes and binds to its cognate operator sequence to repress promoter activity. Upon light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates gene expression.
SD7
A SD sequence upstream of LexRO encoding gene. SD7 is a ribosome binding site on the genome , which is capable of recruiting ribosomes in engineered E. coli.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 456
- 1000COMPATIBLE WITH RFC[1000]