Difference between revisions of "Part:BBa K5175041:Design"
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| − | <i>Pseudomonas aeruginosa</i> | + | rhlA, rhlB are from <i>Pseudomonas aeruginosa</i>. |
===References=== | ===References=== | ||
Latest revision as of 12:06, 2 October 2024
T7 promoter-rhlA-rhlB-T7 terminator
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 827
Illegal PstI site found at 1811
Illegal PstI site found at 1925 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2310
Illegal PstI site found at 827
Illegal PstI site found at 1811
Illegal PstI site found at 1925 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 176
Illegal BamHI site found at 736
Illegal XhoI site found at 912
Illegal XhoI site found at 2159 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 827
Illegal PstI site found at 1811
Illegal PstI site found at 1925 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 827
Illegal PstI site found at 1811
Illegal PstI site found at 1925
Illegal NgoMIV site found at 1052
Illegal NgoMIV site found at 1773
Illegal NgoMIV site found at 1886 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is a composite component consisting of the T7 promoter, lac operator, T7 terminator, target genes rhlA, rhlB.
rhlA, rhlB are from Pseudomonas aeruginosa.
Source
rhlA, rhlB are from Pseudomonas aeruginosa.
References
[1] PATRAUCHAN MARIANNA A, FLORIZONE C, DOSANJH M, et al. Catabolism of Benzoate and Phthalate in Rhodococcus sp. Strain RHA1: Redundancies and Convergence [J]. Journal of Bacteriology, 2005, 187(12): 4050-63.
[2] JARVIS F G, JOHNSON M J. A Glyco-lipide Produced by Pseudomonas Aeruginosa [J]. Journal of the American Chemical Society, 1949, 71(12): 4124-6.
[3] ZHU K, ROCK CHARLES O. RhlA Converts β-Hydroxyacyl-Acyl Carrier Protein Intermediates in Fatty Acid Synthesis to the β-Hydroxydecanoyl-β-Hydroxydecanoate Component of Rhamnolipids in Pseudomonas aeruginosa [J]. Journal of Bacteriology, 2008, 190(9): 3147-54.