Difference between revisions of "Part:BBa K5175042:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | It is a composite component consisting of the T7 promoter, lac operator, T7 terminator, target genes phaZ. <br> | |
| − | + | PhaZ encoded by phaZ is a poly(3-hydroxyalkanoate) depolymerase. | |
Revision as of 07:31, 2 October 2024
T7 promoter-phaZ-T7 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 985
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 492
Illegal NgoMIV site found at 693 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
It is a composite component consisting of the T7 promoter, lac operator, T7 terminator, target genes phaZ.
PhaZ encoded by phaZ is a poly(3-hydroxyalkanoate) depolymerase.
Source
phaZ is from Pseudomonas putida.
References
[20] WITTGENS A, TISO T, ARNDT T T, et al. Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440 [J]. Microb Cell Fact, 2011, 10: 80.
[21] LIU H, CHEN Y, ZHANG Y, et al. Enhanced production of polyhydroxyalkanoates in Pseudomonas putida KT2440 by a combination of genome streamlining and promoter engineering [J]. International Journal of Biological Macromolecules, 2022, 209: 117-24.
[22] 尹进, 车雪梅, 陈国强. 聚羟基脂肪酸酯的研究进展 [J]. 生物工程学报, 32(6): 726-37.