Difference between revisions of "Part:BBa K5127024:Design"
 
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===Design Notes===
 
===Design Notes===
−
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.
+
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in <i>E.coli</i>.
  
  

Latest revision as of 10:04, 2 October 2024


iacR-pIacR-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 732
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.


Source

N/A


References

d’Oelsnitz, S., Stofel, S. K., Love, J. D., & Ellington, A. D. (2024). Snowprint: a predictive tool for genetic biosensor discovery. Communications Biology, 7(1). https://doi.org/10.1038/s42003-024-05849-8

Liang, C., Xiong, D., Zhang, Y., Mu, S., & Tang, S.-Y. (2014). Development of a novel uric-acid-responsive regulatory system in Escherichia coli. Applied Microbiology and Biotechnology, 99(5), 2267–2275. https://doi.org/10.1007/s00253-014-6290-6

Wei, W., Liu, Y., Hou, Y., Cao, S., Chen, Z., Zhang, Y., Cai, X., Yan, Q., Li, Z., Yuan, Y., Wang, G., Zheng, X., & Hao, H. (2023). Psychological stress-induced microbial metabolite indole-3-acetate disrupts intestinal cell lineage commitment. Cell Metabolism. https://doi.org/10.1016/j.cmet.2023.12.026