Difference between revisions of "Part:BBa K5233006:Design"
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===References=== | ===References=== | ||
| + | Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244. | ||
Latest revision as of 02:46, 2 October 2024
tpiA, a kind of tripartite tricarboxylate transporter(TTT) family protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 437
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 437
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1519
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 437
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 437
Illegal NgoMIV site found at 1473
Illegal AgeI site found at 718 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA. .
Source
It comes from Comamonas thiooxidans strain S23 genomic sequence, we obtained it from NCBI
References
Dierkes RF, Wypych A, Pérez-García P, Danso D, Chow J, Streit WR. An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl Environ Microbiol. 2023 Jan 31;89(1):e0160322. doi: 10.1128/aem.01603-22. Epub 2022 Dec 12. PMID: 36507653; PMCID: PMC9888244.