Difference between revisions of "Part:BBa K5233007:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA | + | Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA. |
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Revision as of 02:14, 2 October 2024
tpiB, a kind of tripartite tricarboxylate transporter(TTT) family protein
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 457
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 457
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 496
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 457
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 457
Illegal AgeI site found at 103 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Wild E. coli has low ability to transfer TPA into its body, so we transformed E. coli with plasmids carrying tpiA, tpiB, and tphC to transport TPA.
Source
It comes from Comamonas thiooxidans strain S23.