Difference between revisions of "Part:BBa K5242008"

Latest revision as of 21:40, 1 October 2024

ogRNA-chk1

This part can be complementarily paired with Chk1 and contains an A-C mismatch site in the middle for the introduction of ADAR editing. And this part is the core part of the sensor RNA used to detect Chk1. The addition of four of these MS2 sequences facilitates ADAR recognition and editing. At the beginning and end of the fragment, we added E2A and LV2A peptides, respectively, allowing both fluorescent proteins to be separated from the intervening peptide throughout sensor RNA translation.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 122
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 102
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 122
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 122
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5242008 short</partinfo>
-This part can be fully complementarily paired with the RNA of Chk1, thereby inducing editing of ADAR, and is the core part used to assemble into a sensor for detecting Chk1. The addition of four of these MS2 sequences facilitates ADAR recognition and editing.At the beginning and end of the fragment, we added E2A and LV2A peptides, respectively, allowing both fluorescent proteins to be separated from the intervening peptide throughout sensorRNA translation.
+This part can be complementarily paired with Chk1 and contains an A-C mismatch site in the middle for the introduction of ADAR editing. And this part is the core part of the sensor RNA used to detect Chk1. The addition of four of these MS2 sequences facilitates ADAR recognition and editing. At the beginning and end of the fragment, we added E2A and LV2A peptides, respectively, allowing both fluorescent proteins to be separated from the intervening peptide throughout sensor RNA translation.
 <!-- Add more about the biology of this part here